Production of Herbicide Resistant Transgenic Lily Plants by Particle Bombardment

Transformation of Lilium longiflorum via particle bombardment and generation of herbicide-resistant plants

Lily species (Lilium spp.) are highly appreciated for their aesthetic value as cut flowers or pot plants and have been among the most important ornamental species worldwide for a long time. Numerous lily breeding programs aim at the establishment of novel phenotypes with higher market values. Until now these programs are based mainly on segregation and selection of natural variants and interspe...

Transgenic plant production using particle bombardment in Egyptian barley

A system for Egyptian barley (Hordeum vulgare L. cv Giza 123) transformation via biolistic bombardment has been established. Immature embryos 1.0-1.5 mm in length were aseptically isolated from barley plants cultivated in the open field. Embryos were then cultured on callus induction medium with scutellum side up. Scutellum derived calli were bombarded with the plasmid pAHC25, containing the ma...

Transgenic Herbicide-Resistant Turfgrasses

Transgenic sorghum plants via microprojectile bombardment.

Transgenic sorghum plants have been obtained after microprojectile bombardment of immature zygotic embryos of a drought-resistant sorghum cultivar, P898012. DNA delivery parameters were optimized based on transient expression of R and C1 maize anthocyanin regulatory elements in scutellar cells. The protocol for obtaining transgenic plants consists of the delivery of the bar gene to immature zyg...

Do Environmental Effects of Herbicide-Resistant GM Plants Differ from Effects of Other Herbicide Resistant Plants?

Genetic modification of crop plants has promoted concerns about potential environmental effects of this new technology. In this essay, I will discuss the environmental effects of genetic modification of crop plants using herbicide resistance as an example. Considering herbicide resistance as an old agricultural trait, it is difficult to find any reason to assess the environmental risks of genet...